Vitamin D ELISA kit is a complete kit for the quantitative determination of 25(OH) Vitamin D3 and 25(OH) Vitamin D2 and was tested in human plasma and serum samples. Nevertheless, it is not species specific.
It is recommended that you read the entire kit insert before proceeding with the assay.
Recent research efforts have shown that Vitamin D levels affect various disease states and are being linked with numerous indicators of well-being in humans. These include bone diseases such as osteoporosis and arthritis, but also additional disease including hypertension, diabetes, cancer and heart disease to name a few. Our Vitamin D ELISA kit offers an alternative to labor intensive and/or costly methods of testing for Vitamin D levels in human plasma and serum.
The transformation to the active form of Vitamin D begins with 7-dehydrocholesterol being acted upon by UV rays from the sun to form parent Vitamin D3. Alternatively, Vitamin D can be ingested as parent Vitamin D2 from various food sources, native or fortified. These parent compounds are transported to the liver and undergo hydroxylation to 25(OH) Vitamin D. This metabolite is then transported to the kidney where it undergoes a second hydroxylation to 1,25(OH)2 Vitamin D, the biologically active form of Vitamin D. It is important to note that levels of Vitamin D metabolites increase proportionately with increased uptake of parent Vitamin D. This combined with the greater half-life and stability of 25(OH) Vitamin D in circulation versus the active form (25 days versus 8 hours) are the reasons that the detection of the 25(OH) Vitamin D metabolite is used as the indicator for total Vitamin D concentration.